NPC

Human

 * Baron Flynt - Claims to be the leader of Pandora's bandits. Headquartered on a large digging machine in the Salt Flats. Former employee of Patricia Tannis.
 * Chuck Durden - Owner of the circle of slaughter located in the Rust Commons West.
 * Commandant Steele - Leader of the Crimson Lance.
 * Crazy Earl - Shut-in who lives in Crazy Earl's Scrapyard, off of Rust Commons West, and has a pet Skag, which he lovingly calls "Skrappy."
 * Dr. Zed - The town doctor in Fyrestone. Later moves to New Haven along with the player.
 * Erik Franks - New Haven resident (who wishes he wasn't) with a noticeable appreciation for pornography. Also a loyal follower of Taylor Kobb.
 * Ernest Whitting - A renowned hunter located just inside the entrance of the Dahl Headlands from the Arid Badlands.
 * Helena Pierce - Settlement administrator based out of New Haven. She has an artificial left arm.
 * Hudson Johns - "Custodian" of the Middle of Nowhere Bounty Board.
 * Jaynis Kobb - Brother of Taylor Kobb. Leader of Jaynistown in Rust Commons East. Exiles his brother from the settlement for unknown reasons.
 * Lucky Zaford - Runs Lucky's Last Chance Watering Hole. Allegedly had relations with Scooter's mother that ended badly for both parties.
 * Marcus Kincaid - Narrator, bus driver, and merchant extraordinaire.
 * Patricia Tannis - Former scientific adviser for the Dahl Corporation. Obsessed with finding the Vault.
 * Scooter - Auto-mechanic who maintains the Catch-a-Ride System on Pandora.
 * Shep Sanders - Friend of Dr. Zed whom needs your help with a few missions.
 * Taylor Kobb - Brother of Jaynis Kobb. Exiled from Jaynistown for unknown reasons.
 * T.K. Baha - A blind widower who lives just outside Fyrestone, near Skag Gully. Also has an artificial leg.
 * Rade Zayben - Owner of the Circle of Death located in the Arid Badlands.
 * Dr. Ned Dr. Zed's not-made-up brother

Robot

 * Claptrap - A small robot that introduces you to the game and will help you along with side missions throughout your stay on Pandora. (There is more than one Claptrap unit, however: see: Claptrap Rescue Missions)

Other

 * Guardian Angel - Mysterious benefactor to the player. Can "see" everything on Pandora through the ECHOnet.